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Hemorrhage is a prime cause of death in civilian and military traumatic injuries, whereby a significant proportion of death and complications occur prior to paramedic arrival and hospital resuscitation. Hence, it is crucial to develop hemostatic materials that are able to be applied by simple processes and allow control over bleeding by inducing rapid hemostasis, non-invasively, until subjects receive necessary medical care. This tutorial review discusses recent advances in synthesis and fabrication of degradable hemostatic nanomaterials and nanocomposites. Control of assembly and fine-tuning of composition of absorbable ( i.e. , degradable) hemostatic supramolecular structures and nanoconstructs have afforded the development of smart devices and scaffolds capable of efficiently controlling bleeding while degrading over time, thereby reducing surgical operation times and hospitalization duration. The nanoconstructs that are highlighted have demonstrated hemostatic efficiency pre-clinically in animal models, while also sharing characteristics of degradability, bioabsorbability and presence of nano-assemblies within their compositions. 

The iron-containing heterodimeric MbnBC enzyme complex plays a central role in the biosynthesis of methanobactins (Mbns), ribosomally synthesized, posttranslationally modified natural products that bind copper with high affinity. MbnBC catalyzes a four-electron oxidation of a cysteine residue in its precursor-peptide substrate, MbnA, to an oxazolone ring and an adjacent thioamide group. Initial studies of MbnBC indicated the presence of both diiron and triiron species, complicating identification of the catalytically active species. Here, we present evidence through activity assays combined with electron paramagnetic resonance (EPR) and Mössbauer spectroscopic analysis that the active species is a mixed-valent, antiferromagnetically coupled Fe(II)Fe(III) center. Consistent with this assignment, heterologous expression of the MbnBC complex in culture medium containing less iron yielded purified protein with less bound iron but greater activity in vitro. The maximally activated MbnBC prepared in this manner could modify both cysteine residues in MbnA, in contrast to prior findings that only the first cysteine could be processed. Site-directed mutagenesis and multiple crystal structures clearly identify the two essential Fe ions in the active cluster as well as the location of the previously detected third Fe site. Moreover, structural modeling indicates a role for MbnC in recognition of the MbnA leader peptide. These results add a biosynthetic oxidative rearrangement reaction to the repertoire of nonheme diiron enzymes and provide a foundation for elucidating the MbnBC mechanism. 

Using the Roemmich‐Gilson Argo data set, this study investigates variability of the Subtropical Underwater (STUW) and eastern Subtropical Mode Water (ESTMW) in the South Pacific during 2004–2020. The STUW volume decreased during 2004–2013 and increased during 2013–2020, while the volume of the ESTMW shows the opposite phase. On interannual time scales, there is also a significant negative correlation in volume between the STUW and ESTMW. This anti‐phase relationship is attributed to changes in their volumetric subduction rates, which are in turn closely related to variability in the mixed layer depth (MLD). ENSO directly contributes to variability of the subduction rates by modifying the MLD. Equatorward propagation of spiciness anomalies is identified along isopycnal surfaces of the STUW and ESTMW cores. These spiciness anomalies in the downstream region are correlated with changes in volume of both water masses, and significant spiciness anomalies can reach the tropical Pacific.

 

Both a quasi‐biennial variability and an overall linearly increasing trend are identified in the Sub‐Antarctic Mode Water (SAMW) subduction rate across the Southern Hemisphere ocean, using the Argo data during 2005–2019. The quasi‐biennial variability is mainly due to variability of the mixed layer depth. Variability of wind stress curl in the SAMW formation regions associated with the Southern Annular Mode plays a critical role in generating the quasi‐biennial variability of the mixed layer depth and consequently the SAMW subduction rates. The SAMW subduction rate across the Southern Hemisphere ocean, long‐term mean totaling 56 Sv, has increased at 0.73 ± 0.65 Sv year⁻¹over the past 15 years. The increase has directly contributed to the observed increase in the total SAMW volume. Much of this increasing trend can be explained by the deepening mixed layers, which in turn are primarily forced by the strengthening westerly winds under an increasing Southern Annular Mode.

 

This study investigates the variability of the Southern Hemisphere super gyre (SHSG), using remotely sensed altimeter measurements, in situ Argo observations, and results from an ocean state estimate of the Consortium for Estimating the Circulation and Climate of the Ocean. Analyses of altimeter data show large trends of sea surface height, and their positive‐negative contrast suggests a strengthening of subtropical gyres in all the three Southern Hemisphere oceans since 1993. Analyses of Argo data and the Estimating the Circulation and Climate of the Ocean estimate indicate that these dynamic signals of southern subtropical gyres extend to at least 2,000 m. The three southern subtropical gyres are interconnected through the Tasman and Agulhas leakages and vary consistently during the period 1993–2016. The Tasman and Agulhas leakages also show an increasing trend of inter‐ocean water exchange with a typical increase of ~2 Sv (1 Sv = 10⁶ m³/s) per decade, indicative of a two‐decade‐long spin‐up of the SHSG. The strengthening and poleward shift of westerly winds are associated with an increasing southern annular mode, which affect the midlatitude and high‐latitude Southern Hemisphere oceans and contribute to the spin‐up of the SHSG.

 

Abstract The global network of gravitational-wave observatories now includes five detectors, namely LIGO Hanford, LIGO Livingston, Virgo, KAGRA, and GEO 600. These detectors collected data during their third observing run, O3, composed of three phases: O3a starting in 2019 April and lasting six months, O3b starting in 2019 November and lasting five months, and O3GK starting in 2020 April and lasting two weeks. In this paper we describe these data and various other science products that can be freely accessed through the Gravitational Wave Open Science Center at https://gwosc.org . The main data set, consisting of the gravitational-wave strain time series that contains the astrophysical signals, is released together with supporting data useful for their analysis and documentation, tutorials, as well as analysis software packages.